Recombinant Cdc23 Monoclonal Antibody (AN301810L)
For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, K-562, Mouse lung Verified Samples in IF: HeLa |
| Dilution | WB 1:500-1:1000, IF 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Cdc23 fragment |
| Abbre | Cdc23 |
| Synonyms | CDC, APC, CUT, ANAPC, CDC23, ANAPC8, APC8, CUT23, Anaphase promoting complex subunit 8, Anaphase-promoting complex subunit 8, Apc 8, Cdc 23, cell division cycle 23, Cell division cycle 23 homolog, Cell division cycle protein 23, Cell division cycle protein 23 homolog, Cyclosome subunit 8 |
| Swissprot | |
| Calculated MW | 69 kDa |
| Observed MW |
69 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytosol, Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology, Cancer |
| Clone No. | A522 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | CDC23, also known as APC8, is a component of the tetratricopeptide repeat (TPR) APC/C sub-complex that also includes APC3 (CDC27) and APC6 (CDC16). APC8 protein associates with APC3 and APC6 to facilitate the recruitment of the APC/C coactivation subunits CDC20 and Cdh1/FZR1. APC8 protein is overexpressed in papillary thyroid cancer and acts as an important regulator of cell cycle progression and cell growth. |
Other Clones
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Other Formats
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Unconjugated
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