Recombinant Cdc27 Monoclonal Antibody (AN301730L)
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For research use only.
| Verified Samples |
Verified Samples in WB: MCF-7, BRL Verified Samples in IHC: Human uterine cancer tissue, Human ovarian cancer tissue Verified Samples in IF: 293T Verified Samples in FCM: K562 |
| Dilution | WB 1:1000-1:5000, IHC 1:200-1:1000, IF 1:50, FCM 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, |
| Applications | WB, IHC, IF, FCM |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Cdc27 fragment |
| Abbre | Cdc27 |
| Synonyms | NUC, APC, CDC, ANAPC, CDC27, ANAPC3, APC3, CDC27Hs, D0S1430E, D17S978E, H-NUC, HNUC, NUC2, Anaphase Promoting Complex 3, Anaphase promoting complex protein 3, Anaphase Promoting Complex Subunit 3, Anaphase-promoting complex subunit 3, APC 3, Cdc 27, CDC27 homolog, Cell Division Cycle 27, Cell division cycle protein 27 homolog, H NUC, Nuc2 homolog |
| Swissprot | |
| Calculated MW | 92 kDa |
| Observed MW |
92 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus, Cytoskeleton |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology |
| Clone No. | A438 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Cell division cycle protein 27 homolog (CDC27) is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. |
Other Clones
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Unconjugated
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