Recombinant CDK2 Monoclonal Antibody (E-AB-81426)
For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat, C6, CHO-K1, Hela Verified Samples in IF: Human tonsil, Hela |
| Dilution | WB 1:1000-1:2000, IHC 1:50-1:100, IF 1:20-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Rat, Hamster |
| Applications | WB, IHC-P, IF |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic peptide of human Cdk2 |
| Abbre | CDK2 |
| Synonyms | CDC28, CDC2A, CDK1, CDK2, CDKN2, Cdc2 related protein kinase, Cdk 2, Cell devision kinase 2, Cell division protein kinase 2, Cyclin dependent kinase 2, Cyclin-dependent kinase 2, MPF, cdc2-related protein kinase, cyclin dependent kinase 2-alpha, kinase Cdc2, p33 pr |
| Swissprot | |
| Calculated MW | 34 kDa |
| Observed MW |
34 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoskeleton, centrosome, Cytosol, Endosome, Nucleus, Cajal body, nucleoplasm, nucleus, Other locations: chromosome, telomeric region, condensed chromosome, cyclin A1-CDK2 complex, cyclin A2-CDK2 complex, cyclin E1-CDK2 complex, cyclin E2-CDK2 complex, cyclin-dependent protein kinase holoenzyme complex, cytoplasm, transcription factor complex, X chromosome, Y chromosome. |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Cancer, Cell Biology, Epigenetics and Nuclear Signaling |
| Clone No. | R04-1D5 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family,CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis,but dispensable for mitosis.It has 2 isoforms produced by alternative splicing. |
Other Clones
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Other Formats
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Unconjugated
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