Recombinant CEACAM1 Monoclonal Antibody (AN301418L)
-
-
-
- +1
For research use only.
| Verified Samples |
Verified Samples in WB: PC-3 (Negative control), HepG2 Verified Samples in IHC: Human colon cancer, Human colon Verified Samples in FCM: PBMCs |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100, FCM 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC, FCM |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human CEACAM1 fragment |
| Abbre | CEACAM1 |
| Synonyms | Biliary Glycoprotein, Carcinoembryonic Antigen-Related Cell Adhesion Molecule, CEACAM, CEACAM1, BGP, BGP1, BGPI, BGP-1, Biliary Glycoprotein 1, Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1, CD66a, NCA-160, Antigen CD66, BGP 1, Biliary glycoprotein adhesion molecule, Carcinoembryonic antigen related cell adhesion molecule 1, carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein), CD66a antigen, CEAM1, meconium antigen 100 |
| Swissprot | |
| Calculated MW | 58 kDa |
| Observed MW |
100-180 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Tags & Cell Markers, Immunology, Cancer, Microbiology |
| Clone No. | A113 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner. Plays a role as coinhibitory receptor in immune response, insulin action and functions also as an activator during angiogenesis. Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner. Promotes populations of T cells regulating IgA production and secretion associated with control of the commensal microbiota and resistance to enteropathogens. |
Other Clones
{{antibodyDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
Unconjugated
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
