Recombinant CGA/hCG alpha Monoclonal Antibody (AN301490L)
For research use only.
| Verified Samples |
Verified Samples in WB: JAR Verified Samples in IHC: Human placenta, Human kidney(Negative tissue) |
| Dilution | WB 1:500-1:2000, IHC 1:200-1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human CGA/hCG alpha fragment |
| Abbre | CGA/hCG alpha |
| Synonyms | CGA, CG-ALPHA, FSHA, GPHA1, GPHa, HCG, LHA, TSHA, Beta-Nerve Growth Factor, Beta-NGF, NGF, NGFB, alpha polypeptide, Anterior pituitary glycoprotein hormones common subunit alpha, CG alpha, Choriogonadotropin alpha chain, Chorionic Gonadotrophin Alpha, Chorionic Gonadotrophin Alpha Subunit, Chorionic gonadotrophin subunit alpha, chorionic gonadotropin, Follicle-stimulating hormone alpha, Follicle-stimulating hormone alpha chain, follicle-stimulating hormone alpha subunit, Follitropin alpha, Follitropin alpha chain, FSH Alpha, FSH-alpha, GLHA, Glycoprotein hormones alpha chain, Glycoprotein Hormones Alpha Polypeptide, GPH Alpha, hyrotropin alpha, LH-alpha, LSH alpha, LSH-alpha, Luteinizing hormone alpha, Luteinizing hormone alpha chain, Lutropin alpha, Lutropin alpha chain, Thyroid Stimulating Hormone Alpha, Thyroid Stimulating Hormone Alpha Chain, Thyroid-stimulating hormone alpha chain, Thyrotropin alpha chain, TSH Alpha, TSH-alpha |
| Swissprot | |
| Calculated MW | 13 kDa |
| Observed MW |
18 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Extracellular region, secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Developmental Biology, Cancer, Metabolism, Neuroscience |
| Clone No. | A185 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Shared alpha chain of the active heterodimeric glycoprotein hormones thyrotropin/thyroid stimulating hormone/TSH, lutropin/luteinizing hormone/LH, follitropin/follicle stimulating hormone/FSH and choriogonadotropin/CG. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways. |
Other Clones
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Other Formats
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Unconjugated
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