Recombinant cGAS Monoclonal Antibody (AN301372L)
For research use only.
| Verified Samples | Verified Samples in WB: HT-29 |
| Dilution | WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human cGAS protein |
| Abbre | cGAS |
| Synonyms | MB21D, C6orf, MB21D1, C6orf150, cGAS, h-cGAS, cyclic GMP-AMP synthase, C6orf150, cGAS, cyclic GMP-AMP synthase, h-cGAS, cGAMP synthase, cGMP Synthase, Chromosome 6 open reading frame 150, Hypothetical protein LOC115004, Mab 21 domain containing 1, Mab-21 domain-containing protein 1, MGC131892, MGC142166, MGC142168, OTTHUMP00000016743, OTTHUMP00000039330, protein MB21D1, Uncharacterized protein C6orf150, M21D1 |
| Swissprot | |
| Calculated MW | 62 kDa |
| Observed MW |
62 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Membrane |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cell Biology |
| Clone No. | 5F10 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Enables several functions, including 2',3'-cyclic GMP-AMP synthase activity; molecular condensate scaffold activity; and phosphatidylinositol-4,5-bisphosphate binding activity. Involved in several processes, including defense response to symbiont; intracellular signal transduction; and regulation of defense response. Located in nuclear body; plasma membrane; and site of double-strand break. Is active in cytosol and nucleus. |
Other Clones
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Unconjugated
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