Recombinant CHD3 Monoclonal Antibody (AN301771L)
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For research use only.
| Verified Samples |
Verified Samples in WB: C6, HeLa Verified Samples in IHC: Human breast Verified Samples in IF: HeLa Verified Samples in FCM: HeLa |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100, IF 1:50, FCM 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, |
| Applications | WB, IHC, IF, FCM |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human CHD3 fragment |
| Abbre | CHD3 |
| Synonyms | CHD, CHD3, Mi-2a, Mi2-ALPHA, ZFH, chromodomain helicase DNA binding protein 3 |
| Swissprot | |
| Calculated MW | 227 kDa |
| Observed MW |
260 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus, Centrosome |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology, Epigenetics and Nuclear Signaling |
| Clone No. | A479 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Chromodomain-helicase-DNA-binding protein 3(CHD3) is an enzyme that is encoded by the CHD3 gene in human. This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD3 is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Mutations in CHD3 gene cause a neurodevelopmental syndrome with macrocephaly and impaired speech and language. |
Other Clones
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Other Formats
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Unconjugated
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