Recombinant Cofilin Monoclonal Antibody (E-AB-81548)
For research use only.
| Verified Samples |
Verified Samples in WB: A549, MCF-7, 293T Verified Samples in IHC: Human lung cancer |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:200, IF 1:50-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P, IF |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic peptide of human Cofilin |
| Abbre | Cofilin |
| Synonyms | 18 kDa phosphoprotein, CFL, CFL 1, CFL1, COF1, Cofilin, Cofilin 1, Cofilin 1 non muscle, Cofilin non muscle isoform, Cofilin-1, HEL-S-15, epididymis secretory protein Li 15, non-muscle isoform, p18 |
| Swissprot | |
| Calculated MW | 19 kDa |
| Observed MW |
19 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus matrix. Cytoplasm>cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide. |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Cancer, Cardiovascular, Neuroscience, Signal Transduction |
| Clone No. | R06-2C0 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility,cytokinesis,and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity,also causing translocation from the nucleus to the cytoplasm. |
Other Clones
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Other Formats
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Unconjugated
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