Recombinant Collagen III Monoclonal Antibody (AN301094L)
For research use only.
| Verified Samples |
Verified Samples in WB: Rat womb Verified Samples in IHC: Human skin tissue, Rat skin tissue |
| Dilution | IHC 1:20-100, WB 1:1000-5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human Collagen III protein |
| Abbre | Collagen III |
| Synonyms | CO3A, COL3A, COL3A1, EDS4A, CO3A1_HUMAN, Collagen alpha-1(III) chain, N-Propeptide Of Type III Procollagen, P3NP, PⅢCP, PⅢNP, PC3, PC-III, PCOL-III, Procollagen III Amino Terminal Propeptide, Type III Procollagen, PIIINP, CO3A1, N-Terminal Procollagen Ⅲ Propeptide, Collagen alpha-1(III) chain, Alpha 1 type III collagen, Alpha1 (III) collagen, autosomal dominant, COL 3A1, Collagen, Collagen alpha 1(III) chain, Collagen alpha-1(Ⅲ) chain, Collagen III alpha 1 chain precursor, Collagen III alpha 1 polypeptide, Collagen type III alpha, Collagen type III alpha 1, Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant), Collagen type III alpha 1 chain, Ehlers Danlos syndrome type IV, fetal, Fetal collagen, N-Propeptide Of Type Ⅲ Procollagen, P3CP, PC-Ⅲ, PCOL-Ⅲ, Procollagen Ⅲ Amino Terminal Propeptide, Type Ⅲ Procollagen, Type III collagen |
| Swissprot | |
| Calculated MW | 138 kDa |
| Observed MW |
150 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Stem Cells |
| Clone No. | 6A3 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Collagen type III alpha 1 chain(COL3A1) Homo sapiens This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. |
Other Clones
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Unconjugated
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