Recombinant COPS3 Monoclonal Antibody (AN301497L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, 293, N2a Verified Samples in IHC: Human colon, Mouse cardiac muscle, Rat cardiac muscle Verified Samples in IF: HeLa |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100, IF 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human COPS3 fragment |
| Abbre | COPS3 |
| Synonyms | SGN, Csn, COPS, COPS3, CSN3, SGN3, Constitutive photomorphogenic homolog subunit 3, COP 9 (constitutive photomorphogenic) subunit 3, COP 9 complex homolog subunit 3, COP 9 complex S3, COP 9 complex subunit 3, COP 9 constitutive photomorphogenic homolog subunit 3, COP 9 homolog, COP 9 signalosome complex subunit 3, COP 9 signalosome subunit 3, COP 9 subunit 3, COP9 (constitutive photomorphogenic) subunit 3, COP9 complex homolog subunit 3, COP9 complex S3, COP9 complex subunit 3, COP9 constitutive photomorphogenic homolog subunit 3, COP9 homolog, COP9 signalosome complex subunit 3, COP9 signalosome subunit 3, COP9 subunit 3, COPS 3, CSN 3, JAB 1 containing signalosome subunit 3, JAB1 containing signalosome subunit 3, JAB1-containing signalosome subunit 3, SGN 3, Signalosome subunit 3 |
| Swissprot | |
| Calculated MW | 44 kDa |
| Observed MW |
44 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus, Signalosome |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Neuroscience, Cell Biology |
| Clone No. | A192 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. |
Other Clones
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