Recombinant COUP-TFII Monoclonal Antibody (AN301980L)

Name: Recombinant COUP-TFII Monoclonal Antibody
Brand: Elabscience
SKU: AN301980L
Price: 249.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Resources

Recombinant COUP-TFII Monoclonal Antibody - 1

All	Size	Price	Qty
	100μL	$ 380.00	- +
	50μL	$ 249.00	- +

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Verified Samples	Verified Samples in WB: MCF-7, K562, PC-12 Verified Samples in IHC: Human testis, Human breast cancer, Mouse testis, Rat testis Verified Samples in ChIP: MCF-7
Dilution	WB 1:500-1:1000, IHC 1:50-1:100, ChIP 6μg/2×10⁶cells
Isotype	IgG, κ
Host	Rabbit
Reactivity	Human, Rat, Mouse
Applications	WB, IHC, ChIP
Clonality	Monoclonal;Recombinant
Immunogen	Peptide. This information is proprietary to PTMab.
Abbre	COUP-TFII
Synonyms	SVP, COT, CHTD, NF-E, COUP transcription factor, Apolipoprotein AI regulatory protein, Apolipoprotein A-I regulatory protein, Nuclear receptor subfamily 2 group F member, TFCOUP, COUP TF, COUP-TF, ARP, NR2F2, ARP1, CHTD4, COUPTFB, COUPTFII, NF-E3, SVP40, TFCOUP2, Apolipoprotein AI regulatory protein 1, Apolipoprotein A-I regulatory protein 1, ARP-1, COT2, COUP TF2, COUP transcription factor 2, COUP transcription factor II, COUP-TF II, COUP-TF2, NR2F1, Nuclear receptor subfamily 2 group F member 2
Swissprot	P24468
Calculated MW	46 kDa
Observed MW	46 kDa Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Nucleus
Concentration	1 mg/mL
Buffer	PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Purification Method	Protein A purified
Research Areas	Cardiovascular, Cell Biology, Neuroscience, Epigenetics and Nuclear Signaling, Signal Transduction, Cancer, Metabolism
Clone No.	A696
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	Ice bag
background	Chicken ovalbumin upstream promoter transcription factor (COUP-TF) belongs to the NR2 subfamily of the nuclear hormone receptor family. COUP-TFI and COUP-TFII are two of the well-characterized members in the NR2 subfamily. These two members are highly conserved in their two zinc-finger DNA binding domains (DBD) and the ligand binding domain (LBD), and function as repressors or activators of downstream target genes to regulate different biological processes. COUP-TFI and II bind to 5'-AGGTCA-3' motif palindromes, either directly or indirectly, through heterodimer formation with other proteins (e.g. RXRs) to regulate downstream target gene expression. COUP-TFI is involved in neuronal development, tissue patterning, and differentiation. COUP-TFII has been shown to be involved in angiogenesis, glucose homeostasis, and mesenchymal cell commitment.