Recombinant COX IV Monoclonal Antibody (AN301774L)

Name: Recombinant COX IV Monoclonal Antibody
Brand: Elabscience
SKU: AN301774L
Price: 249.0 USD
Availability: InStock

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Recombinant COX IV Monoclonal Antibody - 1

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	100μL	$ 380.00	- +
	50μL	$ 249.00	- +

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Verified Samples	Verified Samples in WB: Jurkat, MEF, C6, Mouse heart Verified Samples in IHC: Human liver, Human kidney Verified Samples in FCM: HeLa, Jurkat Verified Samples in IP: Jurkat cells extracts
Dilution	WB 1:500-1:2000, IHC 1:100-1:500, FCM 1:50-1:100, IP 1:25-1:50
Isotype	IgG, κ
Host	Rabbit
Reactivity	Human, Rat, Mouse
Applications	WB, IHC, FCM, IP
Clonality	Monoclonal;Recombinant
Immunogen	Recombinant human COX IV fragment
Abbre	COX IV
Synonyms	COX, Cytochrome c oxidase subunit 4 isoform, COX4I, COX4I1, COX IV-1, COX4, COX4-1, COXIV, COXIV-1, COX IV, m, Cytochrome c oxidase subunit 4 isoform 1, COX IV, COX IV-1, Cox4, COX4I1, COXIV, Cytochrome c oxidase subunit 4 isoform 1, AL024441, COX 4, COX IV 1, COX41, Cox4a, COX4B, COX4I2, COX4L2, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial, Cytochrome C Oxidase subunit IV, Cytochrome c oxidase subunit IV isoform 1, Cytochrome c oxidase subunit IV isoform 2 (lung), Cytochrome c oxydase subunit 4, dJ857M17.2, MGC105470, MGC72016, mitochondrial
Swissprot	P13073
Calculated MW	20 kDa
Observed MW	15 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Mitochondrion
Concentration	1 mg/mL
Buffer	PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Purification Method	Protein A purified
Research Areas	Metabolism, Tags & Cell Markers, Signal Transduction, Isotype, Loading Controls, Cancer
Clone No.	A482
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	Ice bag
background	Cytochrome c oxidase IV (COX4) is component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. COX4 is a nuclear-encoded subunit of COX that may play a role in regulating COX activity. COX4 is expressed ubiquitously in adult human tissue with the strongest levels of expression in the pancreas and moderate expression levels in heart, skeletal muscle and placenta.