Recombinant CPS1 Monoclonal Antibody (AN301906L)

Name: Recombinant CPS1 Monoclonal Antibody
Brand: Elabscience
SKU: AN301906L
Price: 249.0 USD
Availability: InStock

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Recombinant CPS1 Monoclonal Antibody - 1

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	100μL	$ 380.00	- +
	50μL	$ 249.00	- +

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Verified Samples	Verified Samples in WB: HeLa, Human liver, Mouse liver, Rat liver Verified Samples in IHC: Human hepatocellular carcinoma, Human liver, Mouse small intestine, Rat liver
Dilution	WB 1:1000-1:5000, IHC 1:50-1:100
Isotype	IgG, κ
Host	Rabbit
Reactivity	Human, Rat, Mouse
Applications	WB, IHC
Clonality	Monoclonal;Recombinant
Immunogen	Recombinant human CPS1 fragment
Abbre	CPS1
Synonyms	CPS, carbamoyl-phosphate synthase, CPSASE, CPS1, CPSASE1, PHN, carbamoyl-phosphate synthase 1, carbamoyl-phosphate synthase 1, CPSASE1, PHN, Carbamoyl phosphate synthase, Carbamoyl phosphate synthase [ammonia], Carbamoyl phosphate synthase [ammonia] mitochondrial, Carbamoyl phosphate synthetase 1, Carbamoyl phosphate synthetase 1 mitochondrial, Carbamoyl phosphate synthetase I, Carbamoylphosphate synthase, Carbamoyl-phosphate synthase [ammonia], Carbamoylphosphate synthetase 1, Carbamoylphosphate synthetase I, Carbamoyl-phosphate synthetase I, CPS 1, CPSase 1, CPSase I, CPSM, mitochondrial, MS738
Swissprot	P31327
Calculated MW	165 kDa
Observed MW	165 kDa Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Mitochondrion
Concentration	1 mg/mL
Buffer	PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Purification Method	Protein A purified
Research Areas	Tags & Cell Markers, Signal Transduction, Metabolism
Clone No.	A622
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	Ice bag
background	Carbamoyl phosphate synthetase 1 (CPS1), a rate-limiting enzyme in the urea cycle, catalyzes the conversion of ammonia and bicarbonate to carbamoyl phosphate in mitochondria. Non-small cell lung carcinoma (NSCLC) cells with oncogenic KRAS and loss of the tumor suppressor LKB1 express CPS1 and depend on this enzyme for growth. CPS1 maintains pyrimidine/purine balance in these cancer cells. Silencing CPS1 reduces the pyrimidine to purine ratio and stalls DNA synthesis, leading to DNA damage and cancer cell death. In addition, the tumor suppressor p53 represses the expression of urea cycle enzymes CPS1, OTC, and ARG1 and, therefore, causes the accumulation of ammonia, suppressing cancer growth. Furthermore, research studies suggest that hypermethylation-mediated downregulation of CPS1 expression may contribute to the progression of normal hepatocytes to hepatocellular carcinoma (HCC).