Recombinant CRMP2 Monoclonal Antibody (AN301738L)
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For research use only.
| Verified Samples |
Verified Samples in WB: SHSY-5Y, NIH-3T3, Mouse brain, Rat brain Verified Samples in IHC: Human colon cancer Verified Samples in IF: HCT116, NIH3T3 |
| Dilution | WB(cell lysate) 1:500-1:2000, WB(tissue lysate) 1:1000-1:10000, IHC 1:200-1:1000, IF 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human CRMP2 fragment |
| Abbre | CRMP2 |
| Synonyms | N2A, DPYSL, DHPRP, DRP, DPYSL2, CRMP-2, CRMP2, DHPRP2, DRP-2, DRP2, N2A3, ULIP-2, ULIP2, dihydropyrimidinase like 2 |
| Swissprot | |
| Calculated MW | 62 kDa |
| Observed MW |
62-66 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell cytosol and membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Neuroscience |
| Clone No. | A446 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Collapsing response mediator protein 2 (CRMP2 and DPYSL2) is known to be a member of the CRMP family. CRMP2 reportedly functions to regulate synaptic transmission, control MT dynamics, and modulate vesicle trafficking in cells and the developing brain. Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis. |
Other Clones
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Other Formats
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Unconjugated
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