Recombinant CSNK2A1 Monoclonal Antibody (AN301754L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat, C6 Verified Samples in IHC: Human breast cancer, Human cerebrum Verified Samples in IF: A549, HepG2 |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:100, IF 1:100-1:200 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, |
| Applications | WB, IHC, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human CSNK2A1 fragment |
| Abbre | CSNK2A1 |
| Synonyms | CK2A, casein kinase 2 alpha, Cka, CK2A1, CKII, CSNK2A3, OCNDS, CKII alpha, CSNK2A1, Cka1, Cka2, casein kinase 2 alpha 1, casein kinase 2 alpha 1, CK2A1, Cka1, Cka2, CKII, CSNK2A3, OCNDS, Casein kinase 2 alpha 1 polypeptide, Casein kinase II alpha 1, Casein kinase II alpha 1 subunit, Casein kinase II alpha subunit, Casein kinase II subunit alpha, CK II, CK II alpha, CK2 alpha, CK2 catalytic subunit alpha, CKIIalpha, CSK21, Csnk2a1-rs4 |
| Swissprot | |
| Calculated MW | 45 kDa |
| Observed MW |
45 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology, Signal Transduction |
| Clone No. | A462 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Casein kinase II subunit alpha (CSNK2A1) is a catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. CSNK2A1 regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. During mitosis, CSNK2A1 functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. |
Other Clones
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