Recombinant Cytokeratin 6 Monoclonal Antibody (AN302351L)
For research use only.
| Verified Samples |
Verified Samples in WB: A431 Verified Samples in IHC: Human tonsil |
| Dilution | IHC 1:200-1:1000, WB 1:2000-1:10000, IF 1:200-1:1000, ELISA 1:5000-1:20000 |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| Clonality | Recombinant;Monoclonal |
| Abbre | Cytokeratin 6 |
| Synonyms | CK 6A, CK 6B, CK 6C, CK 6D, CK 6E, CK-6B, CK-6C, CK-6E, Cytokeratin 6a, Cytokeratin 6B, Cytokeratin 6C, Cytokeratin 6D, Cytokeratin 6E, Cytokeratin-6B, Cytokeratin-6C, Cytokeratin-6E, K2C6C_HUMAN, K6a keratin, K6b keratin, K6C, K6c keratin, K6d keratin, K6e keratin, Keratin, Keratin K6h, Keratin type II cytoskeletal 6A, Keratin type II cytoskeletal 6B, Keratin type II cytoskeletal 6C, Keratin type II cytoskeletal 6D, Keratin type II cytoskeletal 6E, Keratin-6C, KRT6A, KRT6B, KRT6C, KRT6D, KRT6E, type II cytoskeletal 6C, Type-II keratin Kb12 |
| Swissprot | |
| Calculated MW | 60 kDa |
| Observed MW |
60 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Clone | C111971 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
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