Recombinant DDX3 Monoclonal Antibody (AN301878L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, MCF-7, NIH/3T3, C6 Verified Samples in IHC: Human breast cancer, Mouse cerebrum Verified Samples in IF: HeLa Verified Samples in IP: HeLa cells extracts |
| Dilution | WB 1:500-1:1000, IHC 1:100-1:500, IF 1:50, IP 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human DDX3 fragment |
| Abbre | DDX3 |
| Synonyms | HLP, MRX, DDX, DDX3X, CAP-Rf, DBX, DDX14, DDX3, HLP2, MRX102 |
| Swissprot | |
| Calculated MW | 73 kDa |
| Observed MW |
73 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane, Nucleus, Cytoplasm |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Epigenetics and Nuclear Signaling, Stem Cells, Developmental Biology |
| Clone No. | A590 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | DDX3, also named as DBX, DDX3X and HLP2, belongs to the DEAD box helicase family and DED1 subfamily. It is ATP-dependent RNA helicase. DDX3 acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 REV RNAs. It is also involved in HIV-1 replication. DDX3 interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. |
Other Clones
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Other Formats
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Unconjugated
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