Recombinant DDX58 Monoclonal Antibody (AN300675L)
For research use only.
| Verified Samples | Verified Samples in WB: Jurkat |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human DDX58 protein |
| Abbre | DDX58 |
| Synonyms | RLR, DDX, SGMRT, DDX58, RIG-I, RIGI, RLR-1, SGMRT2, DEAD (Asp Glu Ala Asp) box polypeptide 58, DEAD (Asp Glu Ala Asp/His) box polypeptide, DEAD box protein 58, DEAD/H (Asp Glu Ala Asp/His) box polypeptide RIG1, DKFZp434J1111, DKFZp686N19181, FLJ13599, Probable ATP dependent RNA helicase DDX58, Probable ATP-dependent RNA helicase DDX58, Retinoic acid inducible gene 1 protein, Retinoic acid-inducible gene 1 protein, Retinoic acid-inducible gene I protein, RIG I, RIG1, Rig-1, RLR 1, RNA helicase, RNA helicase RIG I |
| Swissprot | |
| Calculated MW | 107 kDa |
| Observed MW |
107 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Epigenetics and Nuclear Signaling, Immunology |
| Clone No. | 6G11 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. |
Other Clones
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Unconjugated
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