Recombinant DNMT3B Monoclonal Antibody (AN302070L)
For research use only.
| Verified Samples |
Verified Samples in WB: HEK-293(Low expression), K562 Verified Samples in ChIP: NCCIT |
| Dilution | WB 1:1000-1:2000, ChIP 6 μg/2×106 cells |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, ChIP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Peptide. This information is proprietary to PTMab. |
| Abbre | DNMT3B |
| Synonyms | DNMT3B, ICF, ICF1, M.HsaIIIB, DNA MTase HsaⅢB|DNA cytosine-5 methyltransferase 3 beta|DNA methyltransferase HsaⅢB, M.HsaⅢB |
| Swissprot | |
| Calculated MW | 96 kDa |
| Observed MW |
96 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Epigenetics and Nuclear Signaling |
| Clone No. | A790 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The DNMT3 family consists of two active methyltransferases, DNMT3A and DNMT3B, along with a DNMT3-like protein known as DNMT3L.DNMT3B plays a crucial role in regulating specific DNA methylation patterns, particularly during de novo methylation. It may function as atranscriptional corepressor by associating with CBX4 independently of DNA methylation. Mutations in the DNMT3B gene lead toImmunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. |
Other Clones
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Unconjugated
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