Recombinant DYNLL1 Monoclonal Antibody (AN301509L)
For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, Neuro-2a, Rat kidney Verified Samples in IHC: Human colon |
| Dilution | WB 1:5000, IHC 1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human DYNLL1 fragment |
| Abbre | DYNLL1 |
| Synonyms | DNCL, hdlc, Dynein Light Chain LC8-Type, DYNLL, DNCLC, DLC, DYNLL1, DLC1, DLC8, DNCL1, DNCLC1, LC8, LC8a, PIN, hdlc1, 8 kDa Dynein Light Chain, Dynein Light Chain 1 Cytoplasmic, Dynein Light Chain LC8-Type 1, Protein Inhibitor of Neuronal Nitric Oxide Synthase, 8kDLC, cytoplasmic, Cytoplasmic dynein light polypeptide, DYL1, Dynein, Dynein light chain 1, Dynein light chain LC8 type 1, HDLC1, LC8-type 1, light chain, light chain 1, light polypeptide 1, MGC126137, MGC126138, MGC72986, Protein inhibitor of neuronal NOS |
| Swissprot | |
| Calculated MW | 10 kDa |
| Observed MW |
10 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Cytoskeleton, Dynein, Microtubule, Mitochondrion, Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology, Neuroscience, Signal Transduction, Epigenetics and Nuclear Signaling, Cancer, Metabolism |
| Clone No. | A208 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures.Binds and inhibits the catalytic activity of neuronal nitric oxide synthase.Promotes transactivation functions of ESR1 and plays a role in the nuclear localization of ESR1.Regulates apoptotic activities of BCL2L11 by sequestering it to microtubules. Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its antiapoptotic activity. |
Other Clones
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Unconjugated
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