Recombinant E-Cadherin/CDH1/E-cad/CD324 Monoclonal Antibody (AN300023P)
For research use only.
| Verified Samples |
Verified Samples in WB: PC-3 in IHC: Human Cholangiocarcinoma in IF: MCF-7 |
| Dilution | WB: 1:1000-1:20000；IHC: 1:200-1:500；ICC/IF: 1:200-1:1000；FC: 1:20 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC, ICC/IF, FC |
| Clonality | Recombinant;Monoclonal |
| Immunogen | Recombinant Human E-cadherin / CDH1 / Cadherin-1 / E-Cad protein |
| Abbre | CDH1 |
| Synonyms | CDH, Arc, CADH, CD antigen CD, Cleaved into: E-Cad/CTF, Cadherin, CAM 120/, Arc-1, CD324, CDHE, ECAD, LCAM, UVO, CDH1, E-Cadherin, CADH1, Cadherin-1, Uvomorulin, E-Cad, CTF2, CAM 120/80, Epithelial cadherin, CD antigen CD324, Cleaved into: E-Cad/CTF1, CTF3, CDH1, Arc 1, Arc 1, CADH1, Cadherin 1, cadherin 1 type 1 E-cadherin, Cadherin1, CAM 120/80, CD 324, CD324, CD324 antigen, cdh1, CDHE, E-Cad/CTF3, E-cadherin, ECAD, Epithelial cadherin, epithelial calcium dependant adhesion protein, LCAM, Liver cell adhesion molecule, UVO, Uvomorulin, Arc-1, Cadherin 1, cadherin 1 type 1 E-cadherin, Cadherin1, CD 324, CD324 antigen, E-Cad/CTF3, epithelial calcium dependant adhesion protein, Liver cell adhesion molecule |
| Swissprot | |
| Calculated MW | 98 kDa |
| Observed MW |
80-120kDa,135 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane, Endosome, Golgi apparatus, Cytoplasm |
| Tissue Specificity | Expressed in granuloma macrophages (at protein level). Expressed in the liver. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Cancer, Developmental Biology, Tags and Cell Markers |
| Clone | A1133 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015] |
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