Recombinant EAAT1 Monoclonal Antibody (AN301072L)
For research use only.
| Verified Samples |
Verified Samples in WB: U-87MG Verified Samples in IHC: Human brain tissue, Mouse brain tissue |
| Dilution | IHC 1:200-1000, WB 1:500-2000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human EAAT1 protein |
| Abbre | EAAT1 |
| Synonyms | EAAT, SLC1A, SLC1A3, EA6, EAAT1, GLAST, GLAST1, EAA1, Excitatory amino acid transporter 1, FLJ25094, GLAST-1, Glial high affinity glutamate transporter, glutamate/aspartate transporter, high affinity, High affinity neuronal glutamate transporter, Sodium dependent glutamate/aspartate transporter, sodium-dependent, Sodium-dependent glutamate/aspartate transporter 1, Solute carrier family 1 (glial high affinity glutamate transporter) member 3, Solute carrier family 1 member 3 |
| Swissprot | |
| Calculated MW | 60 kDa |
| Observed MW |
59 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Signal Transduction, Stem Cells |
| Clone No. | 1F12 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants. |
Other Clones
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Other Formats
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Unconjugated
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