Recombinant EAAT3 Monoclonal Antibody (AN302009L)
For research use only.
| Verified Samples |
Verified Samples in WB: Human heart,?Human liver Verified Samples in IHC: Human kidney, Human oligodendroglioma |
| Dilution | WB 1:1000, IHC 1:500-1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Peptide. This information is proprietary to PTMab. |
| Abbre | EAAT3 |
| Synonyms | EAAC, EAAT, SCZD, SLC1A, SLC1A1, DCBXA, EAAC1, EAAT3, SCZD18, EAA3, EAAC 1, EAAT 3, Excitatory amino acid carrier 1, Excitatory amino acid carrier1, Excitatory amino acid transporter 3, Excitatory amino acid transporter3, Excitatory amino-acid carrier 1, GLUTAMATE TRANSPORTER, HIGH-AFFINITY, MEAAC 1, MEAAC1, member 1, Neuronal and epithelial glutamate transporter, REAAC 1, REAAC1, Slc1 a1, Slc1a 1, Sodium dependent glutamate/aspartate transporter 3, Sodium-dependent glutamate/aspartate transporter 3, Solute carrier family 1, Solute carrier family 1 (neuronal / epithelial high affinity glutamate transporter, Solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, SOLUTE CARRIER FAMILY 1 (NEURONAL/EPITHELIAL HIGH AFFINITY GLUTAMATE TRANSPORTER), Solute carrier family 1 member 1, system Xag) |
| Swissprot | |
| Calculated MW | 57 kDa |
| Observed MW |
70 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane, Recycling endosome membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Neuroscience |
| Clone No. | A729 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | During neurotransmission, glutamate is released from vesicles of the presynaptic cell, and glutamate receptors (e.g., NMDA Receptor, AMPA Receptor) bind glutamate for activation at the opposing postsynaptic cell. Excitatory amino acid transporters (EAATs) regulate and maintain extracellular glutamate concentrations below excitotoxic levels. In addition, glutamate transporters may limit the duration of synaptic excitation by an electrogenic process in which the transmitter is cotransported with three sodium ions and one proton, followed by countertransport of a potassium ion. Five EAATs (EAAT1-5) have been identified. EAAT1 and EAAT2 are expressed mainly in glia, while EAAT3, EAAT4, and EAAT5 are considered to be neuronal transporters. EAAT3 is found in the perisynaptic areas and cell bodies of glutamatergic and GABAergic neurons. Research studies have implicated abnormal EAAT3 expression in the pathophysiology of Schizophrenia. |
Other Clones
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