Recombinant EGFR Monoclonal Antibody (AN300843L)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela Verified Samples in IHC: Mouse salivary gland |
| Dilution | IHC 1:200-1:1000, WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human EGFR protein |
| Abbre | EGFR |
| Synonyms | PIG, HER, Proto-oncogene c-ErbB, Receptortyrosine-protein kinase erbB, Receptor tyrosine-protein kinase erbB, NISBD, EGFR, ERBB, ERBB1, HER1, NISBD2, PIG61, mENA, ErbB-1, Receptortyrosine-protein kinase erbB-1, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, NISBD2, epidermal growth factor receptor, ERBB, ERBB1, HER1, mENA, NISBD2, PIG61, Avian erythroblastic leukemia viral (v erb b) oncogene homolog, Cell growth inhibiting protein 40, Cell proliferation inducing protein 61, EGF R, Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog), Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian), ErbB1, ERBB, erb-b2 receptor tyrosine kinase 1, Errp, Oncogen ERBB, Receptor tyrosine protein kinase ErbB 1, SA7, Species antigen 7, Urogastrone, v-erb-b Avian erythroblastic leukemia viral oncogen homolog, wa2, Wa5 |
| Swissprot | |
| Calculated MW | 134 kDa |
| Observed MW |
175 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Tissue Specificity | Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Cancer |
| Clone No. | 5H2 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. |
Other Clones
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