Recombinant EID1 Monoclonal Antibody (AN302033L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HeLa,?HepG2 Verified Samples in IHC: Human testis, Human kidney, Human cervical cancer |
| Dilution | WB 1:3000, IHC 1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Peptide. This information is proprietary to PTMab. |
| Abbre | EID1 |
| Synonyms | CRI, RBP, Eid, PTD, PNAS, C15orf, C15orf3, CRI1, RBP21, EID1, PNAS-22, PTD014, 21 kDa pRb associated protein, 21 kDa pRb-associated protein, CBP, Chromosome 15 open reading frame 3, CREBBP/EP300 inhibitor 1, CREBBP/EP300 inhibitory protein 1, CRI 1, E1A like inhibitor of differentiation, E1A-like inhibitor of differentiation 1, EID 1, EID-1, EP300 interacting inhibitor of differentiation 1, EP300-interacting inhibitor of differentiation 1, IRO45620, MGC138883, MGC138884, NB4 apoptosis related protein, PNAS 22, PNAS22, Rb and p300 binding protein EID 1, Rb and p300 binding protein EID1, Retinoblastoma protein associated protein |
| Swissprot | |
| Calculated MW | 21 kDa |
| Observed MW |
24 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology, Epigenetics and Nuclear Signaling, Signal Transduction, Cancer |
| Clone No. | A753 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | EID1(EP300 interacting inhibitor of differentiation 1) is a member of the EID protein family, which regulates differentiation, transcription and acetyltransferase activity. EID1 function was originally identified as a transcriptional repressor which regulates cell cycle and differentiation, and leads to genome inactivation and instability by interacting with E3 ubiquitin ligase MDM2. EID1 is mainly located in the cytoplasm. It was translocated into the nuclei under stressed or pathological conditions such as AD patients and APP Tg mice brains. |
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