Recombinant ELP4 Monoclonal Antibody (AN301770L)
For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, NIH-3T3, BRL Verified Samples in IF: HepG2 Verified Samples in IP: HeLa cells extracts |
| Dilution | WB 1:500-1:1000, IF 1:50, IP 1:25-1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IF, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human ELP4 fragment |
| Abbre | ELP4 |
| Synonyms | ELP, Elongator complex protein, hELP, C11orf, dJ68P15A, dJ68P15A.1, Elongation protein 4 homolog, elongation protein 4 homolog (S. cerevisiae), Elongator complex protein 4, FLJ20498, hELP4, PAX6 neighbor gene protein, PAX6NEB, C11orf19, PAXNEB, ELP4, dJ68P15A.1 |
| Swissprot | |
| Calculated MW | 47 kDa |
| Observed MW |
53, 59kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell nucleus and cytosol |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Epigenetics and Nuclear Signaling |
| Clone No. | A478 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Elongator Acetyltransferase Complex Subunit 4 (ELP4) is a multiprotein complex associated with the RNA polymerase II (Pol II) holoenzyme, and which is involved in transcriptional elongation involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. The elongator complex catalyzes formation of carboxy methyluridine in the wobble base at position 34 in tRNAs. Diseases associated with ELP4 include Aniridia 2 and Coloboma of Optic Nerve. Among its related pathways are Mesodermal Commitment Pathway and Chromatin organization. |
Other Clones
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Unconjugated
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