Recombinant EpCAM Monoclonal Antibody (AN300999L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HCT-116 Verified Samples in IHC: Human liver tissue, Mouse colon tissue, Rat prostate |
| Dilution | IHC 1:200-1:1000, WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human EpCAM protein |
| Abbre | EpCAM |
| Synonyms | M4S, MIC, M1S, DIAR, TROP, HNPCC, GA733, TACSTD, EGP, EPCAM, DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1, Ep-CAM, epithelial cell adhesion molecule, GA733-2, M1S2, MK-1, KSA, EGP40, TROP1, 17 1A, 323/A3, 35-KD glycoprotein, Adenocarcinoma associated antigen, Adenocarcinoma-associated antigen, Antigen identified by monoclonal AUA1, AUA1, CD326, CD326 antigen, Cell surface glycoprotein Trop 1, Cell surface glycoprotein Trop 2, Cell surface glycoprotein Trop-1, chromosome 4, CO 17A, CO17 1A, CO17A, EGP 2, EGP2, Ep CAM, EpCAM1, Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD), Epithelial cell surface antigen, Epithelial cellular adhesion molecule, Epithelial glycoprotein, Epithelial glycoprotein 1, Epithelial glycoprotein 314, EpithelialGlycoprotein, GA733 1, GA733 2, gastrointestinal tumor-associated antigen 2, gp4, hEGP 2, hEGP314, Human epithelial glycoprotein 2, KS 1/4 antigen, KS 1/4 antigen.KSA, Ly74, Lymphocyte antigen 74, M1S 1, Major gastrointestinal tumor associated protein GA733 2, Major gastrointestinal tumor-associated protein GA733-2, mEGP314, Membrane component, Membrane component chromosome 4 surface marker (35kD glycoprotein), MK 1, Protein 289A, surface marker, surface marker 1, TACD1, Tumor associated calcium signal transducer 1, Tumor associated calcium signal transducer 2 precursor, Tumor-associated calcium signal transducer 1 |
| Swissprot | |
| Calculated MW | 35 kDa |
| Observed MW |
40 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Tags & Cell Markers |
| Clone No. | 12B5 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. |
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Unconjugated
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