Recombinant EPHB4 Monoclonal Antibody (AN301515L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HT-29, HUVEC, MCF-7 Verified Samples in IF: HT-29, MCF-7 Verified Samples in FCM: HT-29 |
| Dilution | WB 1:500-1:1000, IF 1:50, FCM 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IF, FCM |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human EPHB4 fragment |
| Abbre | EPHB4 |
| Synonyms | MYK, EPHB, TYRO, EPHB4, HFASD, HTK, MYK1, TYRO11 |
| Swissprot | |
| Calculated MW | 108 kDa |
| Observed MW |
120 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cardiovascular, Neuroscience, Stem Cells, Signal Transduction, Cancer |
| Clone No. | A214 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | EPHB4 together with its cognate ligand/functional ligand EFNB2 they are involved in the regulation of cell adhesion and migration, and play a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. |
Other Clones
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Other Formats
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Unconjugated
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