Recombinant FABP7 Monoclonal Antibody (AN301861L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Mouse spleen(-), Mouse brain Verified Samples in IHC: Human cerebrum, Mouse cerebrum, Mouse kidney(Negative tissue) |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human FABP7 fragment |
| Abbre | FABP7 |
| Synonyms | FABP, Fatty Acid-Binding Protein, FABP7, B-FABP, BLBP, FABPB, MRG, Brain Lipid-Binding Protein, Brain-Type Fatty Acid-Binding Protein, Fatty Acid-Binding Protein 7, Fatty Acid-Binding Protein Brain, Mammary-Derived Growth Inhibitor Related, B FABP, BFABP, brain, Brain lipid binding protein, DKFZp547J2313, FABP 7, Fatty Acid Binding Protein 7, Fatty acid binding protein 7 brain, Fatty acid binding protein brain, Mammary derived growth inhibitor related, OTTHUMP00000017119 |
| Swissprot | |
| Calculated MW | 15 kDa |
| Observed MW |
13 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Neuroscience, Signal Transduction, Metabolism |
| Clone No. | A573 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | BLBP, also named as FABP7, is a member of the FABP family of lipid chaperones involved in the uptake and intracellular trafficking of fatty acids. BLBP is expressed in radial glial cells during brain development. BLBP is up-regulated in brain tumor tissue; its expression has been associated with decreased survival times and tumor progression in patients with astrocytoma, melanoma, basal-type breast cancer, and renal cell carcinoma. |
Other Clones
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Unconjugated
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