Recombinant FER Monoclonal Antibody (AN301713L)
For research use only.
| Verified Samples | Verified Samples in WB: HeLa, NIH-3T3 |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human FER fragment |
| Abbre | FER |
| Synonyms | TYK, PPP1R, FER, PPP1R74, TYK3, p94-Fer, c FER, Feline encephalitis virus related kinase FER, Fer (fps/fes related) tyrosine kinase, Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94), FerT, Fujinami poultry sarcoma/Feline sarcoma related protein Fer, p94 FER, Pe1Fe10, Pe1Fe13, Pe1Fe3, Pe1Fe6, Phosphoprotein NCP94, Protein phosphatase 1 regulatory subunit 74, Proto oncogene tyrosine protein kinase FER, Proto-oncogene c-Fer, Tyrosine kinase 3, Tyrosine-protein kinase Fer |
| Swissprot | |
| Calculated MW | 93 kDa |
| Observed MW |
100 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus, Plasma membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology, Signal Transduction |
| Clone No. | A421 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a member of the FPS/FES family of non-transmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. |
Other Clones
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Other Formats
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Unconjugated
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