Recombinant FGFR3 Monoclonal Antibody (AN301784L)
For research use only.
| Verified Samples |
Verified Samples in WB: HepG2, HEK-293, K562, MCF-7 Verified Samples in IP: HEK-293 cells extracts |
| Dilution | WB 1:1000-1:2000, IP 1:25-1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human FGFR3 fragment |
| Abbre | FGFR3 |
| Synonyms | CEK, JTK, Fibroblast growth factor receptor, ACH, CD333, CEK2, HSFGFR3EX, JTK4, FGFR3, FGFR-3, Fibroblast growth factor receptor 3, IIIc, ACH, CD333, FGFR3, Fibroblast growth factor receptor 3, HSFGFR3EX, JTK4, CD 333, CD333 antigen, CEK 2, FGFR 3, Fibroblast growth factor receptor 3 (achondroplasia thanatophoric dwarfism), Heparin binding growth factor receptor, Hydroxyaryl protein kinase, JTK 4, MFR 3, SAM 3, Tyrosine kinase JTK 4, Tyrosine kinase JTK4, Z FGFR 3 |
| Swissprot | |
| Calculated MW | 88 kDa |
| Observed MW |
125 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cardiovascular, Signal Transduction, Cancer, Stem Cells |
| Clone No. | A492 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Fibroblast growth factor receptor 3 is a member of the fibroblast growth factor receptor (FGFR) family, with its amino acid sequence being highly conserved between members and among divergent species. The FGFR proteins are involved in a wide array of pathways known to play a significant role in cancer. Activation of these receptors can lead to activation of the RAS-MAPK pathway and the PI3K-AKT pathway, among others. |
Other Clones
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Other Formats
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Unconjugated
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