Recombinant FOXC1 Monoclonal Antibody (AN301947L)
For research use only.
| Verified Samples | Verified Samples in WB: 293T, MDA-MB-231 |
| Dilution | WB 1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human FOXC1 fragment |
| Abbre | FOXC1 |
| Synonyms | IHG, FOXC, ASGD, FKHL, IRID, RIEG, FOXC1, ARA, ASGD3, FKHL7, FREAC-3, FREAC3, IGDA, IHG1, IRID1, RIEG3, FKH L7, FKHL 7, Forkhead (Drosophila) like 7, Forkhead box C1, Forkhead box protein C1, Forkhead drosophila homolog like 7, Forkhead like 7, Forkhead related activator 3, Forkhead related protein FKHL7, Forkhead related transcription factor 3, Forkhead-related protein FKHL7, Forkhead-related transcription factor 3, FOX C1, FOXC 1, FREAC 3, IHG 1, IRID 1, Iridogoniodysgenesis type 1, Myeloid factor delta |
| Swissprot | |
| Calculated MW | 70-74 kDa |
| Observed MW |
70 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Epigenetics and Nuclear Signaling, Developmental Biology |
| Clone No. | A663 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | FoxC1, also named as FKHL7 or FREAC3, is one of two mammalian FoxC subfamily members. Along with FoxC2, it is expressed in paraxial mesoderm where it functions to promote somitogenesis, myogenesis, and vascular development, possibly under Wnt/β-catenin regulation. Mutations in FoxC1 are implicated in anterior segment dysgenesis (ASD) disorders, including congenital glaucoma and Axenfeld-Rieger syndrome. Research studies have shown that alterations in FoxC1 expression are linked to breast cancer invasiveness and have been shown to modulate proliferation and migration of breast cancer cells in vitro. |
Other Clones
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Unconjugated
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