Recombinant FSH beta Monoclonal Antibody (AN301699L)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse pituitary, Rat pituitary Verified Samples in IHC: Human pituitary, Rat pituitary |
| Dilution | WB 1:500-1:2000, IHC 1:200-1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human FSH beta fragment |
| Abbre | FSH beta |
| Synonyms | FSHB, HH24, beta polypeptide, follicle stimulating hormone, Follicle stimulating hormone subunit beta, Follicle-Stimulating Hormone Beta Subunit, follitropin, Follitropin Beta Chain, Follitropin beta chain precursor, Follitropin Subunit Beta, FSH B, FSH beta, FSH-B, FSH-Beta, FSH, FSHβ |
| Swissprot | |
| Calculated MW | 15 kDa |
| Observed MW |
23 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Cancer, Metabolism, Developmental Biology |
| Clone No. | A407 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Follitropin is involved in follicle development and spermatogenesis in reproductive organs. FSHB together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. FSHB binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. |
Other Clones
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Other Formats
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Unconjugated
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