Recombinant FUBP1 Monoclonal Antibody (AN301527L)
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- +7
For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, K562, Jurkat, Rat brain, Mouse brain Verified Samples in IHC: Human pancreas, Mouse cerebrum, Rat stomach Verified Samples in IF: HeLa, NIH-3T3 Verified Samples in FCM: HeLa, Jurkat Verified Samples in IP: HeLa cells extracts |
| Dilution | WB 1:500-1:2000, IHC 1:200-1:1000, IF 1:50, FCM 1:50-1:100, IP 1:25-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF, FCM, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human FUBP1 fragment |
| Abbre | FUBP1 |
| Synonyms | FUBP1, FBP, FUBP, hDH V, hDHV |
| Swissprot | |
| Calculated MW | 68 kDa |
| Observed MW |
74 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Epigenetics and Nuclear Signaling |
| Clone No. | A226 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The distal upstream original binding protein 1 (FUBP1) is a single-stranded DNA-binding protein that can sometimes bind to single-stranded RNA. FUBP1 is a transactivator of the proto-oncogene c-myc transcription process, that is, FUBP1 binds to the upstream element FUSE of the housekeeping gene c-myc promoter to enhance the transcription of c-myc, thereby promoting cell proliferation, and in the formation of tumors Has an important role. |
Other Clones
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Other Formats
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Unconjugated
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