Recombinant Gemin 2 Monoclonal Antibody (AN301753L)
For research use only.
| Verified Samples | Verified Samples in WB: HepG2, U-2 OS, F9, Rat testis |
| Dilution | WB 1:500-1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Gemin 2 fragment |
| Abbre | Gemin 2 |
| Synonyms | SIP, GEMIN, SIP1, GEMIN2, Component of gems 2, Gem (nuclear organelle) associated protein 2, Gem associated protein 2, GEMI2, Gemin-2, SIP 1, SIP-1, SIP1 delta, SIP1-delta, SMN interacting protein 1, SMN interacting protein 1 delta, SMN-interacting protein 1, Survival interacting protein 1, Survival of motor neuron protein interacting protein 1, Survival of motor neuron protein-interacting protein 1 |
| Swissprot | |
| Calculated MW | 32 kDa |
| Observed MW |
32 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Neuroscience |
| Clone No. | A461 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Gem-associated protein 2 (Gemin 2), also called survival of motor neuron protein-interacting protein 1 (SIP1), is a protein that in humans is encoded by the GEMIN2 gene. Gemin 2 has been shown to interact with DDX20 and SMN1. The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. |
Other Clones
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Unconjugated
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