Recombinant Glutamate receptor 3 Monoclonal Antibody (AN301945L)
For research use only.
| Verified Samples | Verified Samples in WB: Mouse brain, Rat brain |
| Dilution | WB 1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Rat, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Glutamate receptor 3 fragment |
| Abbre | Glutamate receptor 3 |
| Synonyms | MRX, GRIA, GLUR, GluA, GLUR-K, GRIA3, GLUR-C, GLUR-K3, GLUR3, GLURC, GluA3, MRX94, AMPA 3, AMPA selective glutamate receptor 3, AMPA-selective glutamate receptor 3, dJ1171F9.1, GLUK3, GluR 3, GLUR C, GLUR K3, GluR-3, Glutamate ionotropic receptor AMPA type subunit 3, Glutamate receptor, Glutamate receptor 3, Glutamate receptor C, Glutamate receptor ionotrophic AMPA 3, Glutamate receptor ionotropic, Glutamate receptor subunit 3, GRIA 3, Ionotrophic Glutamate Receptor, ionotropic |
| Swissprot | |
| Calculated MW | 101 kDa |
| Observed MW |
101 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Neuroscience, Signal Transduction |
| Clone No. | A661 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4) that assemble as homo- or hetero-tetramers and mediate the majority of fast excitatory transmissions in the CNS. AMPARs are implicated in synapse formation, stabilization and plasticity. Glutamate receptor 3 (GluR3) knockout mice exhibited normal basal synaptic transmission and long-term depression (LTD) but enhanced long-term potentiation (LTP). In contrast, GluR 2/3 double knockout mice are impaired in basal synaptic transmission. Aberrant GluR3 expression or activity is implicated in a number of diseases, including autoimmune epilepsy, X-linked mental retardation, Rett's syndrome, amyotrophic lateral sclerosis and Alzheimer disease. |
Other Clones
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Unconjugated
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