Recombinant GOT2 Monoclonal Antibody (AN301538L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, HepG2, PC-12, Mouse kidney Verified Samples in IHC: Human kidney, Mouse liver, Rat stomach |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human GOT2 fragment |
| Abbre | GOT2 |
| Synonyms | KAT, GOT, KYAT, GOT2, KAT4, KATIV, KYAT4, mitAAT, AATM, AL022787, Aspartate aminotransferase, Aspartate aminotransferase 2, Aspartate transaminase 2, ASPATA, EC 2.6.1.1, FABP 1, FABP pm, FABP-1, FABPpm, Fatty acid binding protein, Fatty acid-binding protein, FLJ40994, Glutamate oxaloacetate transaminase, Glutamate oxaloacetate transaminase 2, Glutamic oxaloacetic transaminase 2, Got 2, kynurenine aminotransferase 4, Kynurenine aminotransferase IV, kynurenine--oxoglutarate transaminase 4, kynurenine--oxoglutarate transaminase IV, mAspAT, MGC102129, MGC115763, mitochondrial, mitochondrial (aspartate aminotransferase 2), Mitochondrial aspartate aminotransferase, OTTMUSP00000017748, Plasma membrane fatty acid binding protein, Plasma membrane-associated fatty acid-binding protein, Transaminase A |
| Swissprot | |
| Calculated MW | 42 kDa |
| Observed MW |
42 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane, Mitochondrion |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Cancer, Metabolism |
| Clone No. | A237 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Aspartate aminotransferase, mitochondrial is an enzyme that in humans is encoded by the GOT2 gene. Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. Also, GOT2 is a major participant in the malate-aspartate shuttle, which is a passage from the cytosol to the mitochondria. The two enzymes are homodimeric and show close homology. GOT2 has been seen to have a role in cell proliferation, especially in terms of tumor growth. |
Other Clones
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Unconjugated
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