Recombinant HER2 Monoclonal Antibody (AN300730L)
For research use only.
| Verified Samples | Verified Samples in WB: SK-BR-3 |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human HER2 protein |
| Abbre | HER2 |
| Synonyms | TKR, ERBB, erb-b2 receptor tyrosine kinase, Receptor tyrosine-protein kinase erbB, Tyrosine kinase-type cell surface receptor HER, HER2/ErbB, HER, MLN, ERBB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1, erb-b2 receptor tyrosine kinase 2, HER2/ErbB2, MLN19, Metastatic lymph node gene 19 protein, p185, Proto-oncogene Neu, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, Neu, erb-B2, CD340, erb-b2 receptor tyrosine kinase 2, HER2, HER-2, HER-2/neu, MLN 19, MLN19, NGL, TKR1, C erb B2/neu protein, CD340 antigen, Cerb B2/neu protein, CerbB2, ENV, ENVW, Erb b2 receptor tyrosine kinase 2, ERVWE1, HER 2, HER 2/NEU, Herstatin, HERV7Q, HERV-7q, HERVW, HERVWENV, HERV-W-ENV, Human epidermal growth factor receptor 2, NEU proto oncogene, neuro/glioblastoma derived oncogene homolog, neuro/glioblastoma derived oncogene homolog (avian), Neuroblastoma/glioblastoma derived oncogene homolog, p185erbB2, Proto-oncogene c-ErbB-2, Tyrosine kinase type cell surface receptor HER2, V erb b2 avian erythroblastic leukemia viral oncogene homolog 2, V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog), V erb b2 avian erythroblastic leukemia viral oncoprotein 2, V erb b2 erythroblastic leukemia viral oncogene homolog 2, Verb b2 erythroblastic leukemia viral oncogene homolog 2 |
| Swissprot | |
| Calculated MW | 138 kDa |
| Observed MW |
185 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Tags & Cell Markers, Signal Transduction, Cancer |
| Clone No. | 6H3 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. |
Other Clones
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Unconjugated
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