Recombinant HIV gp120 Protein (His Tag) (PKSQ050061)
 
                        For research use only.
| Synonyms | Envelope glycoprotein gp120, Glycoprotein 120, Surface protein gp120 | 
| Species | HIV | 
| Expression Host | HEK293 Cells | 
| Sequence | Met1-Glu498 | 
| Accession | Q9DKG6 | 
| Calculated Molecular Weight | 57.0 kDa | 
| Observed Molecular Weight | 90-140 kDa | 
| Tag | C-His | 
| Bio-activity | Not validated for activity | 
| Form | Lyophilized powder | 
| Purity | > 95 % as determined by reducing SDS-PAGE. | 
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. | 
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. | 
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. | 
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. | 
| Reconstitution | Please refer to the printed manual for detailed information. | 
| Background | Envelope glycoprotein gp160 is single-pass type I membrane protein. The mature envelope protein (Env) consists of a homotrimer of non-covalently associated gp120-gp41 heterodimers. It is cleaved into the following 2 chains: glycoprotein 120 and transmembrane protein gp41. The resulting complex protrudes from the virus surface as a spike. The 17 amino acids long immunosuppressive region is present in many retroviral envelope proteins. Synthetic peptides derived from this relatively conserved sequence inhibit immune function in vitro and in vivo. | 
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