Recombinant HIV p24 protein (C-6His) (PKSV030277)
For research use only.
| Synonyms | Capsid protein p24, Human immunodeficiency virus 1, Human immunodeficiency virus type 1 p24 |
| Species | HIV |
| Expression Host | E.coli |
| Sequence | Met142-Gly355 |
| Accession | P12493 |
| Calculated Molecular Weight | 24.6 kDa |
| Observed Molecular Weight | 25 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 5% Trehalose, 5% Mannitol,0.01% Tween 80, 1mM EDTA, pH 9.0. |
| Reconstitution | Not Applicable |
| Background | The HIV1 p24 protein performs highly complex orchestrated tasks during the assembly,budding maturation and infection stages of the viral replication cycle. During viral assembly,the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag p24, the major capsid protein of the HIV-1 virion, has been used in clinical trials as one of the components of the HIV-1 vaccine because of the high degree of sequence conservation between different isolates. Recombinant HIV1-p24 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography. |
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