Recombinant HLA-DPB1 Monoclonal Antibody (AN301551L)
-
-
-
- +2
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse spleen, Raji Verified Samples in IHC: Human tonsil Verified Samples in IF: Raji, Ramos Verified Samples in IP: Raji cells extracts |
| Dilution | WB 1:500-1:1000, IHC 1:200-1:1000, IF 1:50, IP 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human HLA-DPB1 fragment |
| Abbre | HLA-DPB1 |
| Synonyms | DPB, DP beta, DPB1, HLA-DP, HLA-DP1B, HLA-DPB, HLA-DPB1, major histocompatibility complex, class II, DP beta 1, beta-1 subunit, class I, D6S221E, DMA, DMB, DP beta 1 chain, DP(W4) beta chain, DPB 1, DRB, H2Ea, HLA class II histocompatibility antigen, HLA class II histocompatibility antigen DM beta chain, HLA DMB, HLA DP1A, HLA DPB1, HLA SB alpha chain, HLA-A, HLA-A histocompatibility type, HLADM, HLA-DP histocompatibility type, HLADP1B, HLASB, HLASB histocompatibility type, Human MHC class II HLA SB alpha, LA class II histocompatibility antigen DP alpha 1 chain, Major histocompatibility complex class II, Major histocompatibility complex class II DP alpha 1, Major histocompatibility complex class II DP beta 1, MHC class II antigen DMB, MHC class II antigen DPB1, MHC class II DP3 alpha, MHC class II DPA1, MHC class II HLA-DP-beta-1, MHC DPB1, MHC HLA DPB1, PLT1, Primed lymphocyte test 1, RING6, RING7 |
| Swissprot | |
| Calculated MW | 29 kDa |
| Observed MW |
29 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology |
| Clone No. | A250 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. |
Other Clones
{{antibodyDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
Unconjugated
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
