Recombinant HMGB1 Monoclonal Antibody (AN300860L)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela Verified Samples in IHC: Mouse liver |
| Dilution | IHC 1:2000-1:10000, WB 1:5000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human HMGB1 protein |
| Abbre | HMGB1 |
| Synonyms | SBP, HMGB, High Mobility Group Protein, High mobility group protein B, HMG, HMG-1, HMG1, HMG3, SBP-1, HMGB1, Amphoterin, High Mobility Group Protein 1, High mobility group protein B1, High mobility group protein 1 (HMG-1), High mobility group protein 1, high mobility group protein B1, high-mobility group box 1, HMG1, HMG-1, HMG1DKFZp686A04236, HMG3, HMGB1, SBP-1, Chromosomal protein, DKFZp686A04236, High mobility group 1, High mobility group box 1, high-mobility group (nonhistone chromosomal) protein 1, HMGB 1, nonhistone, NONHISTONE CHROMOSOMAL PROTEIN HMG1, SBP 1, Sulfoglucuronyl carbohydrate binding protein |
| Swissprot | |
| Calculated MW | 25 kDa |
| Observed MW |
25 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Epigenetics and Nuclear Signaling |
| Clone No. | 7B7 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | HMGB1(high mobility group box 1) Homo sapiens. This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. |
Other Clones
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Other Formats
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Unconjugated
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