Recombinant HSP90 beta Monoclonal Antibody (AN301558L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat, PC-12, Mouse testis, Rat testis Verified Samples in IHC: Human testis, Mouse cerebrum, Rat cerebrum Verified Samples in IP: HeLa cells extracts |
| Dilution | WB 1:10000-1:50000, IHC 1:50-1:100, IP 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human HSP90 fragment |
| Abbre | HSP90 beta |
| Synonyms | D6S, HSPC, HSP90AB, HSP, D6S182, HSP84, HSP90B, HSPC2, HSPCB, Hsp90 beta, HSP90AB1, heat shock protein HSP 90-beta, Hsp90, D6S182, heat shock protein HSP 90-beta, HSP84, HSP90B, HSPC2, HSPCB, 90 kda heat shock protein beta HSP90 beta, beta, FLJ26984, Heat shock 84 kDa, Heat shock 90kD protein 1, Heat shock 90kDa protein 1 beta, Heat shock protein 90 alpha family class B member 1, Heat shock protein 90 kDa, Heat shock protein 90kDa alpha (cytosolic) class B member 1, Heat shock protein 90kDa alpha family class B member 1, Heat shock protein beta, Heat shock protein HSP 90 beta, HS90B, HSP 84, HSP 90, HSP 90 b, HSP 90b |
| Swissprot | |
| Calculated MW | 85 kDa |
| Observed MW |
90 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Membrane, Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Cancer |
| Clone No. | A257 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Hsp90 (90 kDa heat shock protein) is a molecular chaperone that aids protein folding and quality control for a large number of 'client' proteins. Hsp90 operates as dimer and has intrinsic ATPase activity. The Hsp90 dimer acts in concert with other chaperones (e.g. Hsp70). |
Other Clones
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Other Formats
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Unconjugated
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