Recombinant Human B4GAT1/B3GNT1 Protein (His Tag) (PKSH032105)
For research use only.
| Synonyms | 3-N-Acetylglucosaminyltransferase, 3-N-Acetylglucosaminyltransferase 1, B3GNT1, B3GNT6, I-Beta-1, N-Acetyllactosaminide Beta-1, Poly-N-Acetyllactosamine Extension Enzyme, UDP-GlcNAc:BetaGal Beta-1, iGnT |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Asp43-Cys415 |
| Accession | O43505 |
| Calculated Molecular Weight | 43.4 kDa |
| Observed Molecular Weight | 45-55 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl,150mM NaCl, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | N-Acetyllactosaminide β-1,3-N-Acetylglucosaminyltransferase (B3GNT1) is a member of the β-1,3-N-Acetylglucosaminyltransferase family. B3GNT1 is a single-pass type II membrane protein and widely expressed in many tissues. B3GNT1 can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans. B3GNT1 is essential for the synthesis of poly-N-acetyllactosamine, a determinant for the blood group i antigen. It can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans. |
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