Recombinant Human D-Amino-Acid Oxidase/DAO Protein (His Tag) (PKSH032342)
For research use only.
| Synonyms | D-Amino-Acid Oxidase, DAAO, DAMOX, DAO |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Leu347 |
| Accession | P14920 |
| Calculated Molecular Weight | 41.6 kDa |
| Observed Molecular Weight | 40 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 20% Glycerol, 1mM DTT, 2mM EDTA, pH 8.0. |
| Reconstitution | Not Applicable |
| Background | D-Amino-Acid Oxidase (DAO) belongs to the DAMOX/DASOX family. DAO is a peroxisomal enzyme which founctions as a homodimer to oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. D-amino-acid oxidase regulates the level of the neuromodulator D-serine in the brain, has a high activity towards D-DOPA and contributes to dopamine synthesis. D-amino-acid oxidase could act as a detoxifying agent which removes D-amino acids accumulated during aging. It also acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. |
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