Recombinant Human FGF-2/FGFb Protein (PKSH032438)
For research use only.
| Synonyms | Basic fibroblast growth factor, Bfgf, FGF-2, FGF2, FGFB, Fibroblast growth factor 2, HBGF-2, Heparin-binding growth factor 2 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ala144-Ser288 |
| Accession | P09038 |
| Calculated Molecular Weight | 18.1 kDa |
| Observed Molecular Weight | 17 kDa |
| Tag | None |
| Bio-activity | Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is <1 ng/mL. The specific activity of recombinant human FGF-2 is approximately >5 x 105 IU/mg. |
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile PBS with 0.01% sarkosyl,pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Fibroblast growth factor 2(FGF2) is a secreted protein and belongs to the heparin-binding growth factors family. FGF2 is produced by epithelial; tumor and other cell types. It involved in developmental processes and regulates differentiation; proliferation; and migration; FGF2 is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF2 has a high affinity for heparan sulfate and binding is a step in the FGF basic activation of FGFR tyrosine kinase. |
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