Recombinant Human Fibronectin/FN Protein (PKSH032450)
For research use only.
| Synonyms | CIG, ED-B, FINC, FN, FN1, FNZ, Fibronectin, GFND, GFND2, LETS, MSF |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Pro1270-Ser1546&Ala1721-Thr2016 |
| Accession | P02751 |
| Calculated Molecular Weight | 62.7 kDa |
| Observed Molecular Weight | 60-80 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.01 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from a 0.2 μm filtered solution of 12.5 mM Citric acid, 1.25% Sucrose, 0.1% Tween 80, pH 5.5 . Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains;fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment; is a protein of ~63 kDa containing a central cell-binding domain; a high affinity heparin-binding domain II;and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand; a CS-I site; and a VLA-5 ligand; a cell attachment domain; and virus vectors binds to a heparin binding domain II; which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors; and facilitates the gene transduction in the result. |
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