Recombinant Human GDF-15 protein (His Tag) (PDEH101053)
For research use only.
| Synonyms | GDF-15, GDF15, Growth/differentiation factor 15 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Gln151-Ile308 |
| Accession | Q99988 |
| Calculated Molecular Weight | 17.3 kDa |
| Observed Molecular Weight | 20 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Background | Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and-9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems. |
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