Recombinant Human HGPRT/HPRT1 Protein (His Tag) (PKSH032542)
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For research use only.
| Synonyms | HGPRT, HGPRTase, HPRT, HPRT1, Hypoxanthine-Guanine Phosphoribosyltransferase |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Ala218 |
| Accession | P00492 |
| Calculated Molecular Weight | 27.8 kDa |
| Observed Molecular Weight | 29 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
| Form | Liquid |
| Purity | > 85 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 250mM NaCl, 50% Glycerol, pH8.0. |
| Background | Hypoxanthine-Guanine Phosphoribosyltransferase (HGPRT) has an important role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 functions to salvage purines from degraded DNA to renewed purine synthesis, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP. |
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