Recombinant Human HLA-A*0201 NY-ESO-1 complex Protein (C-10His) (PKSH034036)
For research use only.
| Synonyms | HLA-A*0201 NY-ESO-1 complex Protein, MHC, MY-ESO-1 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | SLLMWITQC&Ile21-Met119&Gly25-Ile308(Ala269Val) |
| Accession | P61769&P01892 |
| Calculated Molecular Weight | 49.1 kDa |
| Observed Molecular Weight | 55-60 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
| Form | Lyophilized powder |
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 500mM NaCl, pH7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | NY-ESO-1 is a well-known cancer-testis antigen (CTAs) with re-expression in numerous cancer types. Its ability to elicit spontaneous humoral and cellular immune responses, together with its restricted expression pattern, have rendered it a good candidate target for cancer immunotherapy. Optimally assembled peptide-HLA-B2M trimer translocates to the surface of antigen-presenting cells, where it interacts with TCR and CD8 coreceptor on the surface of T cells. |
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